Adult neurogenesis and neuronal regeneration in the brain of teleost fish


Progeny of adult stem cells isolated from the brain of the teleost fish, Apteronotus leptorhynchus. Differentiation is induced by plating neurospheres onto a laminin‑coated surface and cultivating them in medium containing fetal bovine serum. Approximately 24 h after plating, the cells start to differentiate into various cell types. (A) GFAP‑immunoreactive astrocyte. (B) Vimentin‑positive cell. (C) Hu C/D‑expressing neurons. (D) Microtubule‑associated protein‑2 (2a + 2b)‑immunopositive neuron. Nuclei are stained with either propidium iodide (red) or 4’‑6‑diamidino‑2‑phenylindole dihydrochloride, DAPI (blue). Note the absence of immunolabeling in some cells in (C) and (D). (From Hinsch & Zupanc, 2006.)

Three-dimensional reconstruction of radial glial fibers in the cerebellum of the teleost fish, Apteronotus leptorhynchus. These fibers play an important role in the guidance of adult-born neurons migrating from proliferation zones to their target areas. (From: Zupanc et al., 2012.)

Selected Publications:

Zupanc, G.K.H., Zupanc, M.M.: Birth and migration of neurons in the central posterior/prepacemaker nucleus during adulthood in weakly electric knifefish, Eigenmannia sp. Proceedings of the National Academy of Sciences U.S.A. 89, 9539-9543 (1992), URL:

Zupanc, G.K.H., Horschke, I.: Proliferation zones in the brain of adult gymnotiform fish: a quantitative mapping study. Journal of Comparative Neurology 353, 213-233 (1995), doi:10.1002/cne.903530205

Zupanc, G.K.H., Horschke, I., Ott, R., Rascher, G.B.: Postembryonic development of the cerebellum in gymnotiform fish. Journal of Comparative Neurology 370, 443-464 (1996), doi:10.1002/(SICI)1096-9861(19960708)370:4<443::AID-CNE3>3.0.CO;2-4

Zupanc, G.K.H., Kompass, K.S., Horschke, I., Ott, R., Schwarz, H.: Apoptosis after injuries in the cerebellum of adult teleost fish. Experimental Neurology 152, 221-230 (1998), doi:10.1006/exnr.1998.6853

Zupanc, G.K.H., Ott, R.: Cell proliferation after lesions in the cerebellum of adult teleost fish: time course of generation, site of origin, and type of new cells produced. Experimental Neurology 160, 78-87 (1999), doi:10.1006/exnr.1999.7182

Zupanc, G.K.H., Hinsch, K., Gage, F.H.: Proliferation, migration, neuronal differentiation, and long-term survival of new cells in the adult brain of zebrafish. Journal of Comparative Neurology 488, 290-319 (2005), doi:10.1002/cne.20571

Zupanc, M.M., Wellbrock, U.M., Zupanc, G.K.H.: Proteome analysis identifies novel protein candidates involved in regeneration of the cerebellum of teleost fish. Proteomics 6, 677-696 (2006), doi:10.1002/pmic.200500167

Hinsch, K., Zupanc, G.K.H.: Isolation, cultivation, and differentiation of neural stem cells from adult fish brain. Journal of Neuroscience Methods 158, 75-88 (2006), doi:10.1016/j.jneumeth.2006.05.020

Hinsch, K., Zupanc, G.K.H.: Generation and long-term persistence of new neurons in the adult zebrafish brain: a quantitative analysis. Neuroscience 146, 679-696 (2007), doi:10.1016/j.neuroscience.2007.01.071

Rajendran, R.S., Zupanc, M.M., Lösche, A., Westra, J., Chun, J., Zupanc, G.K.H.: Numerical chromosome variation and mitotic segregation defects in the adult brain of teleost fish. Developmental Neurobiology 67, 1334-1347 (2007), doi:10.1002/dneu.20365

Rajendran, R.S., Wellbrock, U.M., Zupanc, G.K.H.: Apoptotic cell death, long-term persistence, and neuronal differentiation of aneuploid cells generated in the adult brain of teleost fish. Developmental Neurobiology 68, 1257-1268 (2008), doi:10.1002/dneu.20656

Zupanc, G.K.H., Wellbrock, U.M., Sîrbulescu, R.F., Rajendran, R.S.: Generation, long-term persistence, and neuronal differentiation of cells with nuclear aberrations in the adult zebrafish brain. Neuroscience 159, 1338-1348 (2009), doi:10.1016/j.neuroscience.2009.02.014

Zupanc, G.K.H., Sîrbulescu, R.F., Ilieş, I.: Radial glia in the cerebellum of adult teleost fish: Implications for the guidance of migrating new neurons. Neuroscience 210, 416-430 (2012), doi:10.1016/j.neuroscience.2012.03.012

Teles, M.C., Sîrbulescu, R.F., Wellbrock, U.M., Oliveira, R.F., Zupanc, G.K.H.: Adult neurogenesis in the brain of the Mozambique tilapia, Oreochromis mossambicus. Journal of Comparative Physiology A 198, 427-449 (2012), doi:10.1007/s00359-012-0721-6

Ilieş, I., Zupanc, M.M., Zupanc, G.K.H.: Proteome analysis reveals protein candidates involved in early stages of brain regeneration in teleost fish. Neuroscience 219, 302-313 (2012), doi:10.1016/j.neuroscience.2012.05.028

Sîrbulescu, R.F., Ilieş, I., Zupanc, G.K.H.: Quantitative analysis reveals dominance of gliogenesis over neurogenesis in an adult brainstem oscillator. Developmental Neurobiology 74, 932-952 (2014), doi:10.1002/dneu.22176

Sîrbulescu, R.F., Ilieş, I., Zupanc, G.K.H.: Matrix metalloproteinase-2 and -9 in the cerebellum of teleost fish: Functional implications for adult neurogenesis. Molecular and Cellular Neuroscience 68, 9-23 (2015), doi:10.1016/j.mcn.2015.03.015

Sîrbulescu, R.F., Ilieş, I., Vitalo, A.G., Trull, K., Zhu, J., Traniello, I.M., Zupanc, G.K.H.: Adult stem cells in the knifefish cerebellum. Developmental Neurobiology 75, 39-65 (2015), doi:10.1002/dneu.22210